Morphological aspects of prevention of postoperative hepatic insufficiency with cyanocobalamin solution
- Authors: Shishkina V.V.1, Laptiyova A.Y.1, Ostroushko A.P.1, Yashkova M.A.1
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Affiliations:
- Voronezh State Medical University named after N.N. Burdenko
- Issue: Vol 11, No 2 (2022): Materials of the XV International Surgical Forum "INNOVATIVE TECHNOLOGIES IN SURGERY"
- Pages: 80-82
- Section: Хирургические дисциплины
- URL: https://new.vestnik-surgery.com/index.php/2415-7805/article/view/7653
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Abstract
Goal. Improving the results of prevention of postoperative hepatic insufficiency by activating the reparative potential of the liver with cyanocobalamin administration.
Materials and methods. The study was performed on 36 Wistar rats. In 3 experimental groups, resection was performed in the amount of 70% of the initial liver mass: in the 1st - prevention of postoperative liver failure was not carried out, in the 2nd - after resection, 0.9% NaCl solution was injected into the preserved liver lobes, in the 3rd - cyanocobalamin. Liver resection was not performed in the 1st control group. The result was evaluated 2 weeks after surgery. The MS Excel package was used for statistical processing.
Results. Histological and immunohistochemical studies 2 weeks after surgery showed the best results in the 3rd experimental group: the number of binucleated hepatocytes was 19.31±3.68%, ki67-positive hepatocyte nuclei 6.22± 0.54%. When studying liver sections stained with silver impregnation, the number of reticular fibers in the 3rd experimental group was more than 90% of the indicators of the 1st control group, in the 1st and 2nd experimental groups – less than 80%.
Conclusion. The proposed method of activating the reparative potential of the liver by the introduction of cyanocobalamin allows to increase the proliferative activity of hepatocytes, contributes to an increase in the number of cells entering the mitotic cycle, ensures the restoration of the structural organization of the liver, its anatomical and functional integrity.
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Relevance. Liver resection in most cases remains one of the optimal ways to achieve an increase in life expectancy and cure of patients [1-5]. As a rule, operations with the removal of three or more segments are used for tumor and parasitic diseases, which in most cases is the cause of severe complications and post-resection liver failure [2-7].
Goal. Improving the results of prevention of postoperative hepatic insufficiency by activating the reparative potential of the liver with cyanocobalamin administration.
Materials and methods. The study was performed on 36 Wistar rats. In 3 experimental groups, resection was performed in the amount of 70% of the initial liver mass: in the 1st - prevention of postoperative liver failure was not carried out, in the 2nd - after resection, 0.9% NaCl solution was injected into the preserved liver lobes, in the 3rd - cyanocobalamin. Liver resection was not performed in the 1st control group. The result was evaluated 2 weeks after surgery. The MS Excel package was used for statistical processing.
Results. In histological and immunohistochemical studies 2 weeks after surgery, the best results were observed in the 3rd experimental group: the number of binucleated hepatocytes was 19.31±3.68%, ki67-positive hepatocyte nuclei 6.22± 0.54%, which exceeded the indicators of the 1st and 2nd experimental groups by 1.5 and 3 times, respectively. When studying liver sections stained with silver impregnation, the number of reticular fibers in the 3rd experimental group was more than 90% of the indicators of the 1st control group, in the 1st and 2nd experimental groups – less than 80%.
Conclusion. The proposed method of activating the reparative potential of the liver by the introduction of cyanocobalamin allows to increase the proliferative activity of hepatocytes, contributes to an increase in the number of cells entering the mitotic cycle, ensures the restoration of the structural organization of the liver, its anatomical and functional integrity.
About the authors
Victoria Viktorovna Shishkina
Voronezh State Medical University named after N.N. Burdenko
Email: 4128069@gmail.com
Director of the Research Institute of Experimental Biology and Medicine, Candidate of Medical Sciences, Associate Professor
Russian Federation, 394036, Russia, Voronezh, 10 Studentskaya str.Anastasia Yurievna Laptiyova
Voronezh State Medical University named after N.N. Burdenko
Author for correspondence.
Email: laptievaa@mail.ru
ORCID iD: 0000-0002-3307-1425
SPIN-code: 7626-9016
Assistant
Russian Federation, 394036, Russia, Voronezh, 10 Studentskaya str.Anton Petrovich Ostroushko
Voronezh State Medical University named after N.N. Burdenko
Email: oap@vrngmu.com
ORCID iD: 0000-0003-3656-5954
SPIN-code: 9811-2385
Candidate of Medical Sciences, Associate Professor
Russian Federation, 394036, Russia, Voronezh, 10 Studentskaya str.Maria Alexandrovna Yashkova
Voronezh State Medical University named after N.N. Burdenko
Email: YshkovaMasha@yandex.ru
Student, Voronezh State Medical University named after N.N. Burdenko
Russian Federation, 394036, Russia, Voronezh, 10 Studentskaya str.References
- Глухов А.А., Андреев А.А., Лаптиёва А.Ю., Остроушко А.П. Первичная профилактика пострезекционной печеночной недостаточности. Профилактическая медицина. 2020. Т. 23. № 5-2. С. 44-45.
- Андреев А.А., Лаптиёва А.Ю., Остроушко А.П. Репаративная регенерации после оперативного лечения первичных опухолей и метастатическом поражении печени. Многопрофильный стационар. 2018. Т. 5. № 2. С. 100-104.
- Назыров Ф., Акбаров М., Девятов А., и др. Результаты резекционных вмешательств на печени на фоне хронической диффузной гепатопатии // Вестник экспериментальной и клинической хирургии. - 2015. - Т. 8. - №2. - C. 142-150.
- Ботиралиев А.Ш., Степанова Ю.А., Вишневский В.А., Чжао А.В. Пострезекционные билиарные осложнения // Вестник экспериментальной и клинической хирургии. - 2021. - Т. 14. - №3. - C. 228-236.
- Лаптиёва А.Ю., Андреев А.А.Оценка функциональной активности резецированной печени при внутрипеченочном введении цианокобаламина. Молодежный инновационный вестник. 2019. Т. 8. № 2. С. 32-34.
- Затолокин В., Перьков А., Лунева Н. Особенности изменения белкового обмена в зависимости от объема резекции печени // Вестник экспериментальной и клинической хирургии. - 2011. - Т. 4. - №2. - C. 340-343.
- Андреев А.А., Шишкина В.В., Лаптиева А.Ю., Глухов А.А., Остроушко А.П.. Особенности пострезекционной регенерации гепатоцитов под влиянием внутрипеченочного введения цианокобаламина. Журнал анатомии и гистопатологии. 2021. Т. 10. № 3. С. 27-34.