Молодежный инновационный вестникМолодежный инновационный вестник2415-7805Федеральное государственное бюджетное образовательное учреждение высшего образования "Воронежский государственный медицинский университет имени Н.Н. Бурденко" Министерства здравоохранения Российской Федерации9935Conference ProceedingsProliferation and apoptosis of rat hepatocytes after local electron irradiationYakimenkoVladislav AndreevichYavladislav87@gmail.comhttps://orcid.org/0000-0003-2308-6313UgurchievaDali Ibragimovnadaliyagurchieva@gmail.comhttps://orcid.org/0009-0004-7308-8450VadyukhinMatvey Anatolyevichvma20@mail.ruhttps://orcid.org/0000-0002-6235-1020DemyashkinGrigory Aleksandrovichdr.dga@mail.ruhttps://orcid.org/0000-0001-8447-2600Sechenov University271220241321431440404202411062024Copyright © 2024, Yakimenko V.A., Ugurchieva D.I., Vadyukhin M.A., Demyashkin G.A.2024<p>Introduction. When malignant neoplasms of the abdominal cavity are irradiated, healthy hepatocytes can also be exposed to toxic ionizing radiation, which can cause disruption of their life cycle, death and the development of signs of acute radiation hepatitis. Target. Immunohistochemical assessment of proliferation and apoptosis of hepatocytes in a model of acute radiation hepatitis. Materials and methods. Experimental animals (Wistar rats) were divided into 2 groups: control and experimental. The latter was subjected to local irradiation with electrons at a dose of 30 Gy. Then, the morphology of liver fragments was studied and immunohistochemical reactions were performed with antibodies to Ki-67 and caspase-3. Results. A week after local irradiation with electrons at a total dose of 30 Gy, a shift in the proliferative-apoptotic ratio towards apoptosis was found, confirmed by an increase in the proportion of caspase-3-positive hepatocytes and a decrease in the number of Ki-67-stained cells compared to the control group. Conclusions. An immunohistochemical study of the proliferation and apoptosis of hepatocytes revealed that a week after fractional irradiation with electrons in an SOD of 30 Gy, there is a decrease in mitotic activity and an increase in cell death, and mild signs of radiation hepatitis indicate a more sparing effect of electrons compared to other types of ionizing radiation.</p>liverhepatocyteapoptosiselectron irradiationimmunohistochemistryпеченьгепатоцитапоптозоблучение электронамииммуногистохимия1.Zhu W, Zhang X, Yu M. Radiation-induced liver injury and hepatocyte senescence. Cell Death Discov. 2021; 7: 244.2.Yang W, Shao L, Zhu S. Transient Inhibition of mTORC1 Signaling Ameliorates Irradiation-Induced Liver Damage. Front Physiol. 2019; 10: 228.3.Abdel-Aziz N, Haroun RA, Mohamed HE. Low-Dose Gamma Radiation Modulates Liver and Testis Tissues Response to Acute Whole Body Irradiation. Dose Response. 2022; 20(2): 15593258221092365.4.Zhou YJ, Tang Y, Liu SJ. Radiation-induced liver disease: beyond DNA damage. Cell Cycle. 2023; 22(5): 506-526.5.Иммуногистохимическая оценка пролиферации и апоптоза кератиноцитов после однократного фракционного ионизирующего облучения / Е. Ю. Шаповалова, А. Х. Марукян, М. А. Вадюхин, Г. А. Демяшкин // Морфологические ведомости. – 2023. – Т. 31, № 3. – С. 23-29. – EDN JPWFFP.