Молодежный инновационный вестникМолодежный инновационный вестник2415-7805Федеральное государственное бюджетное образовательное учреждение высшего образования "Воронежский государственный медицинский университет имени Н.Н. Бурденко" Министерства здравоохранения Российской Федерации9934Conference ProceedingsLife cycle assessment in rat ovarian follicles after local electron irradiationPugachevaEkaterina Nilolaevnarouzella@mail.ruhttps://orcid.org/0009-0009-2268-3838MurtazalievaZaira MagomedovnaZARIA.ALIEVA.90@BK.RUhttps://orcid.org/0009-0000-2361-7618UgurchievaDali Ibragimovnadaliyagurchieva@gmail.comhttps://orcid.org/0009-0004-7308-8450VadyukhinMatvey Anatolyevichvma20@mail.ruhttps://orcid.org/0000-0002-6235-1020DemyashkinGrigory Aleksandrovichdr.dga@mail.ruhttps://orcid.org/0000-0001-8447-2600Sechenov UniversirySechenov University271220241321061070404202418062024Copyright © 2024, Pugacheva E.N., Murtazalieva Z.M., Ugurchieva D.I., Vadyukhin M.A., Demyashkin G.A.2024<p>Introduction. The topic of reproductive dysfunction in patients undergoing radiation treatment for malignant neoplasms of the pelvic organs remains poorly studied. Particularly interesting is the experimental study of disturbances in the life cycle of ovarian follicles as a result of exposure to local electron irradiation, which is a relatively new and promising method of radiotherapy. Aim. Immunohistochemical assessment of the life cycle of ovarian follicles in a model of radiation-induced ovarian failure. Materials and methods. Experimental animals (Wistar rats) were divided into 2 groups: control and experimental. The experimental group was locally irradiated with electrons. Then, fragments of the ovaries were examined by immunohistochemistry with antibodies to the proliferation factors Ki-67 and apoptosis termination caspase-3. Results. In the group of local electron irradiation with a total dose of 20 Gy on the 7th day of the experiment, a statistically significant increase in the proportion of caspase-3-positive granulosa cells and a decrease in their proliferative activity were found according to the results of immunohistochemical reactions. The proportion of single stained cells of the corpus luteum and thecal membrane was practically no different from the indicators in the control group. Conclusions. Local irradiation with electrons in a total dose of 20 Gy leads to a slight imbalance of markers of the life cycle of the granulosa membrane in radiation-induced ovarian failure. Low indicators of the range and depth of post-radiation lesions of the ovary indicate a more gentle effect of electrons, and a more detailed study of this type of ionizing radiation can be useful in the development of new regimens and indications for prescribing electron therapy in the practice of a radiobiologist.</p>ovarygranulosa cellslife cycleelectron irradiationimmunohistochemistryяичникгранулезные клеткижизненный циклоблучение электронамииммуногистохимия1.Ahmed Y., Khan A.M.H., Rao U.J., et al. Fertility preservation is an imperative goal in the clinical practice of radiation oncology: a narrative review. Ecancermedicalscience. 2022;16:1461.2.An J., Du X., Zhang F., et al. Effect of radiotherapy on ovarian function in patients with cervical cancer undergoing radical surgery. Chinese Journal of Radiation Oncology. 2019;28(10):753-756.3.Reisz J.A., Bansal N., Qian J., et al. Effects of ionizing radiation on biological molecules--mechanisms of damage and emerging methods of detection. Antioxidants Redox Signals. 2014;21(2):260-292.4.Calvo FA, Serrano J, Cambeiro M, et al. Intra-Operative Electron Radiation Therapy: An Update of the Evidence Collected in 40 Years to Search for Models for Electron-FLASH Studies. Cancers (Basel). 2022;14(15):3693.5.Влияние факторов роста тромбоцитов на пролиферацию сперматогенного эпителия после локального облучения электронами в нестерилизующей дозе / Г. А. Демяшкин, Т. Г. Боровая, Ю. Ю. Андреева [и др.] // Вестник Российского научного центра рентгенорадиологии. – 2021. – Т. 21, № 4. – С. 51-68. – EDN SMOPGY.