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<article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" article-type="research-article" dtd-version="1.1d1" xml:lang="en"><front><journal-meta><journal-id journal-id-type="publisher">Молодежный инновационный вестник</journal-id><journal-title-group><journal-title>Молодежный инновационный вестник</journal-title></journal-title-group><issn publication-format="print">2415-7805</issn><publisher><publisher-name>Федеральное государственное бюджетное образовательное учреждение высшего образования "Воронежский государственный медицинский университет имени Н.Н. Бурденко" Министерства здравоохранения Российской Федерации</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="publisher-id">9778</article-id><article-categories><subj-group subj-group-type="heading"><subject>Conference Proceedings</subject></subj-group></article-categories><title-group><article-title>Selection and optimisation of nutrient media and conditions for in vitro tissue culture of the lichen Eviernia prunastri (l.) Ach.</article-title></title-group><contrib-group><contrib contrib-type="author"><name name-style="western"><surname>Suleymanova</surname><given-names>Anife Renatovna</given-names></name><email>a.suleymanova017@gmail.com</email><uri content-type="orcid">https://orcid.org/0009-0005-6962-6372</uri><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author"><name name-style="western"><surname>Kiriakidi</surname><given-names>Eleonora Pavlovna</given-names></name><bio>&lt;p&gt;Lecturer of the Medical College of the Order of the Red Banner of Labour of the S.I. Georgievsky Medical Institute of the V.I. Vernadsky Crimean Federal University&lt;/p&gt;</bio><email>kiriakidi98@list.ru</email><uri content-type="orcid">https://orcid.org/0009-0000-3222-9481</uri><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author"><name name-style="western"><surname>Egorova</surname><given-names>Elena Alexandrovna</given-names></name><bio>&lt;p&gt;Candidate of Pharmaceutical Sciences, Assistant Professor, Department of Basic and Clinical Pharmacology, Order of the Red Banner of Labour, S.I. Georgievsky Medical Institute of the V.I. Vernadsky Crimean Federal University&lt;/p&gt;</bio><email>egorovapharm@mail.ru</email><uri content-type="orcid">https://orcid.org/0000-0003-4012-2523</uri><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff id="aff-1">V.I. Vernadsky Crimean Federal University</aff><pub-date date-type="epub" iso-8601-date="2024-04-19" publication-format="electronic"><day>19</day><month>04</month><year>2024</year></pub-date><volume>13</volume><issue>S1</issue><fpage>596</fpage><lpage>598</lpage><history><pub-date date-type="received" iso-8601-date="2024-02-21"><day>21</day><month>02</month><year>2024</year></pub-date><pub-date date-type="accepted" iso-8601-date="2024-05-24"><day>24</day><month>05</month><year>2024</year></pub-date></history><permissions><copyright-statement>Copyright © 2024, Suleymanova A.R., Kiriakidi E.P., Egorova E.A.</copyright-statement><copyright-year>2024</copyright-year></permissions><abstract>&lt;p&gt;&lt;em&gt;Abstract.&lt;/em&gt;&lt;/p&gt;&#13;
&lt;p&gt;&lt;em&gt;Introduction. In the process of selection and optimisation of nutrient media and in vitro cultivation conditions, morphological, cyto- and histochemical analyses of E. prunastri lichen cells and tissues were performed. In order to create optimal conditions for lichen cultivation, lichen growth was carried out on different variants of nutrient media: liquid, solid and with the addition of alcoholic extracts or aqueous decoctions from plants on which E. prunastri grows in natural conditions. Materials and methods: the study was conducted using E. prunastri lichen plants collected in natural habitats of the Crimean peninsula, using the in vitro tissue culture method. Results: the optimal conditions for growth and development of lichen E. prunastri (L.) Ach were determined during the study. Conclusion: based on the obtained data, a number of regularities at the morpho-, cyto- and histochemical levels were identified, which contribute to the creation of the most favourable nutrient medium for cultivation of the studied plant.&lt;/em&gt;&lt;/p&gt;</abstract><kwd-group xml:lang="en"><kwd>moss, breeding ground, optimization of nutrient mediums, cultivation in vitro, tissue culture</kwd></kwd-group><kwd-group xml:lang="ru"><kwd>лишайник</kwd><kwd>питательные среды</kwd><kwd>оптимизация питательных сред</kwd><kwd>культивирование in vitro</kwd><kwd>культура тканей</kwd></kwd-group></article-meta></front><body></body><back><ref-list><ref id="B1"><label>1.</label><mixed-citation>Теплицкая Л.М., Семенова Е.Ф., Кириакиди Э.П. Цито- и гистохимические исследования лишайника Еvernia prunastri (l.) Ach. Семейства Usneaceae в культуре in vitro // Биология растений и садоводства: теория и инновации, 2021 г. – C. 156</mixed-citation></ref><ref id="B2"><label>2.</label><mixed-citation>Теплицкая Л.М., Семенова Е.Ф. Биотехнология получения резиноида из клеточных культур Evernia prunastri // Материалы III Международной научно-практической конференции «Современные проблемы отечественной медико-биологической и фармацевтической промышленности», Пенза, 21 ноября 2014 г. – Пенза: ИИЦ ПГУ, 2014. – С. 160-173</mixed-citation></ref><ref id="B3"><label>3.</label><mixed-citation>Семенова Е.Ф., Гаврилова Е.Н., Преснякова Е.В. Фармацевтический словарь-cправочник по биотехнологии. - Пенза: Изд-во ПГУ, 2014. - 147 с.</mixed-citation></ref><ref id="B4"><label>4.</label><mixed-citation>Теплицкая Л.М., Семенова Е.Ф. Биотехнология получения резиноида из клеточных культур Evernia prunastri // Материалы III Международной научно-практической конференции «Современные проблемы отечественной медико-биологической и фармацевтической промышленности», Пенза, 21 ноября 2014 г. Пенза: ИИЦ ПГУ, 2014. - С. 160-173.</mixed-citation></ref><ref id="B5"><label>5.</label><mixed-citation>Сафонова, М. Ю. Фармакогностическое и фармакологическое изучение слоевищ цетрарии исландской Cetraria islandica (L.) Ach.: автореф. дис. канд. фарм. наук (15.00.02, 14.00.25) / М. Ю. Сафонова. - СПб., 2018. - 27 с.</mixed-citation></ref></ref-list></back></article>
