Молодежный инновационный вестникМолодежный инновационный вестник2415-7805Федеральное государственное бюджетное образовательное учреждение высшего образования "Воронежский государственный медицинский университет имени Н.Н. Бурденко" Министерства здравоохранения Российской Федерации8785Conference ProceedingsBIOCRYSTALLOMICS AS A METHOD OF PREDICTING THE COURSE OF REPAIR OF BURN WOUNDS OF THE SKINBuglakAnna Olegovnasnejo4ik@gmail.comhttps://orcid.org/0009-0005-0534-7921ShestakovaValeria Gennadievnashestvg@mail.ruhttps://orcid.org/0000-0003-1136-7396GaninaEkaterina Borisovnakatena-ganina@mail.ruhttps://orcid.org/0000-0002-8686-8526Tver State Medical University2903202312124251504202315042023Copyright © 2023, Buglak A.O., Shestakova V.G., Ganina E.B.2023<p style="text-align: justify;">In pathological processes, there is a change in the chemical composition of tissues and body fluids, which can fix it by crystallizing the object under study. The change in the chemical composition of plasma is important in the dynamics of the skin regeneration process. The aim of the study was a qualitative and quantitative assessment of the plasma crystallization process and the determination of the dependence of the crystallomorphological pattern on the timing of repair and the change of phases of healing of burn wounds. The study was conducted on 12 white mongrel rats kept in the same vivarium conditions, with a standard maintenance regime. Thermal burn on the dorsal surface of the body was simulated in rats. Blood plasma was taken for the study on the 7th, 14th, 21st days of the experiment. The plasma obtained from experimental animals was compared with the plasma of an intact rat. A 2% solution of ninhydrin was used as a diagnostic solution. Stereometric processing of the results consisted in calculating the density of crystallization centers. As a result, crystals of different sizes and shapes were obtained. According to the results of the study, it can be concluded that plasma crystallomorphology can be used as one of the additional methods of investigation and diagnosis of regeneration processes of burn wounds.</p>burn woundrepaircrystallomorphologybiocrystallomicsbiological fluidожоговая ранарепарациякристалломорфологиябиокристалломикабиологическая жидкость<p style="text-align: justify;">Relevance. When studying the features of plasma crystallization, it was found that it can serve as an objective indicator of the functional state of the organism.</p> <p style="text-align: justify;">The purpose of the study. Qualitative and quantitative assessment of the plasma crystallization process and determination of the dependence of the crystallomorphological pattern on the timing of repair and the change of phases of healing of burn wounds.</p> <p style="text-align: justify;">Materials and methods. The study was conducted on 12 male mongrel white rats. All experimental studies were conducted in compliance with the rules and regulations for working with experimental animals [1].Thermal burns with a total area of 225 mm2 were applied to the animals on the pre-prepared dorsal surface of the body using a special soldering iron. Plasma was taken from animals on the 7th, 14th, 21st days of the experiment.For diagnosis, 2 ml of plasma was taken, to which a two percent alcohol solution of ninhydrin (10 ml) was added. The mixture was poured into a Petri dish and left for 12-14 hours to crystallize. Then the crystals were examined under a light microscope and photographed.The crystallized plasma of an intact rat was used as a reference.Stereometric processing of the results consisted in calculating the density of crystallization centers. Nine visual fields were viewed on the measured surface in two mutually perpendicular directions and the number of crystallization centers within each field was calculated [2].</p> <p style="text-align: justify;">Results. When the alcohol solution of ninhydrin is crystallized without additives, large crystals are formed in the form of regular spherulites. The shape and size of the crystals changed when rat plasma was added to the alcohol solution of ninhydrin at different stages of repair.</p> <p style="text-align: justify;">Conclusions. Plasma crystallomorphology can be used as one of the additional methods of investigation and diagnosis of regeneration processes of burn wounds.</p>1.Донсков С. А. Техника безопасности при работе студентов в гистологической лаборатории в рамках учебного процесса / С. А. Донсков, Е. Б. Ганина, В. Г. Шестакова // «Актуальные проблемы безопасности жизнедеятельности и экологии»: материалы II Международной научно-практической конференции с научной школой для молодежи Минобр и науки РФ ФГБОУ ВО «Тверской государственный технический университет» (24-27 марта 2016 года). – Тверь, 2016. - С. 253-254.2.Курбатова Л.А. Кристаллизация в биологических средах //Тезисы докладов.Тверь. ТГМИ. -1992. - С.16.