Молодежный инновационный вестникМолодежный инновационный вестник2415-7805Федеральное государственное бюджетное образовательное учреждение высшего образования "Воронежский государственный медицинский университет имени Н.Н. Бурденко" Министерства здравоохранения Российской Федерации8482UnclassifiedTHE ISSUE OF DIAGNOSING AUTISTIC SPECTRUM DISORDER IN CHILDREN WITH GASTROINTESTINAL DYSFUNCTIONSIakovenkoAnnna Evgenievna<p>student</p>tidal_wave_@mail.ruhttps://orcid.org/0000-0002-1226-1764St. Petersburg State Pediatric Medical University2004202312S23473501702202328022023Copyright © 2023, Iakovenko A.E.2023<p><span style="font-weight: 400;">The </span><strong>aim of the research</strong><span style="font-weight: 400;"> is to examine the state of the parietal microbiome in children with ASD using gas chromatography-mass spectrometry of blood microbial markers, to determine the likely triggers of ASD in children with functional disorders of the gastrointestinal tract.</span></p> <p></p> <p><strong>Materials and methods</strong><span style="font-weight: 400;">. Small intestine microbiome was studied by chromatography-mass spectrometry of a dry blood drop (CMS according to G.A. Osipov). 39 children (1.5 - 10 years old) took part in the research. All children had stable functional gastrointestinal disorders. The first group (26 children) included children without signs of autism and other psycho-neurological dysfunctions. Children from the second group (13 people) were previously diagnosed with ASD in accordance with CARS scale by a psychiatrist. Statistical data were processed on a PC using the STATISTICA 12 software package. ROC analysis was performed.</span></p> <p></p> <p><strong>Results</strong><span style="font-weight: 400;">. The state of the microbiome in the first group significantly differs from the second group. Children diagnosed with ASD show predominance of Clostridium ramosum, Clostridium perfringens, Eggerthella lenta and Nocardia asteroides. Moreover, the amount of Clostridium ramosum in children diagnosed with ASD as well as suffering from functional disorders of the gastrointestinal tract, in more than 75% of cases exceeds the reference values (2000 10 * 5 cells / gram). The average value of Clostridium ramosum for this group is 3494 10*5 cells/gram. Thus, the study of the parietal microbiota in children with signs of autistic disorders can be used as a method of additional diagnostics.</span></p> <p><br /><strong>Conclusions</strong><span style="font-weight: 400;">. One of the most significant differences in microbiome of autistic and neurotypical children with gastrointestinal symptoms is the overgrowth of Clostridium ramosum, which may be used as a factor in the early diagnosis of ASD in children.</span></p>intestine's microbiota and ASDmicrobom in childrendiagnosis of the ASDgastrointestinal symptoms in childrenfunctional disorders of the gastrointestinal tract in childrenexcessive clostridial growthClostridium ramosumмикробиота кишечника и РАСмикробом у детейдиагностика РАСгастроинтенстинальные симптомы у детейфункциональные нарушения ЖКТ у детейизбыточный клостридиальный ростClostridium ramosum1.Gyawali S, Patra BN. Autism spectrum disorder: Trends in research exploring etiopathogenesis. Psychiatry ClinNeurosci. 2019 Aug;73(8):466-475. doi: 10.1111/pcn.12860.2.JohnsonD, LetchumananV, ThurairajasingamS, LeeLH. A Revolutionizing Approach to Autism Spectrum Disorder Using the Microbiome. Nutrients. 2020 Jul 3;12(7):1983. doi: 10.3390/nu12071983.3.Kang D.W., Park J.G., Ilhan Z.E., Wallstrom G., LaBaer J., Adams J.B., et al. Reduced incidence of Prevotella and other fermenters in intestinal microflora of autistic children. PLoS One. 2013; 8(7): e68322. https://doi.org/10.1371/journal.pone.00683224.Madra M, Ringel R, Margolis KG. Gastrointestinal Issues and Autism Spectrum Disorder. Child Adolesc Psychiatr Clin N Am. 2020 Jul;29(3):501-513. doi: 10.1016/j.chc.2020.02.005.5.Meng-Chuan Lai, Michael V Lombardo, Simon Baron-Cohen. Autism. Lancet. 2014 Mar 8;383(9920):896-910. doi: 10.1016/S0140-6736(13)61539-16.Saurman V, Margolis KG, Luna RA. Autism Spectrum Disorder as a Brain-Gut-Microbiome Axis Disorder. DigDisSci. 2020 Mar;65(3):818-828. doi: 10.1007/s10620-020-06133-5.7.Осипов Г.А., Бойко Н.Б., Новикова В.П., Гриневич В.Б., Федосова Н.Ф., Цех О.М., Токарева Е.В., Земскова Е.А. Методика масс-спектрометрии микробных маркеров как способ оценки пристеночной кишечной микробиоты при заболеваниях органов пищеварения. Санкт-Петербург, 2013.