Молодежный инновационный вестникМолодежный инновационный вестник2415-7805Федеральное государственное бюджетное образовательное учреждение высшего образования "Воронежский государственный медицинский университет имени Н.Н. Бурденко" Министерства здравоохранения Российской Федерации10859UnclassifiedCharacterisation of changes in the expression of the Eda2r gene under the influence of metformin in different tissues of the aged ratSidorovaDaria Nikolaevnadora.s.02@inbox.ruhttps://orcid.org/0009-0002-6020-9618EgorovaEmilia Sergeevnajastspring@yandex.ruhttps://orcid.org/0000-0002-6210-4660ValeevaElena Valeryevnavevaleeva@ya.ruhttps://orcid.org/0000-0001-7080-3878AkhmetovIldus Ilyasovichgenoterra@mai.ruhttps://orcid.org/0000-0002-6335-4020Kazan State Medical University041120251421311351404202507082025Copyright © 2025, Sidorova D.N., Egorova E.S., Valeeva E.V., Akhmetov I.I.2025<p style="text-align: justify;"><span style="font-weight: 400;">The study of molecular mechanisms underlying aging and the action of geroprotective agents represents a highly relevant area of biomedical research. This work investigates the effects of intragastric administration of metformin (35 and 70 mg/kg) on the expression of the Eda2r gene in various tissues of aged Wistar rats of both sexes. Gene expression analysis was performed using real-time PCR in 10 tissue types (brain, muscle, adipose tissue, liver, heart) and whole blood following 30-days of treatment. In male rats, metformin at a dose of 35 mg/kg induced a 30-fold increase in Eda2r expression in the quadriceps femoris muscle, whereas in females a 7-fold decrease was observed at a dose of 70 mg/kg. Additionally, a significant reduction in Eda2r expression was detected in the cardiac tissue of females. Sex-specific differences in Eda2r expression were observed both in control groups and in response to metformin, along with distinct tissue-specific coexpression patterns. These findings suggest that Eda2r is involved in tissue- and sex-specific regulation under aging conditions and highlight its potential as a molecular marker in gerontological research.</span></p>agingexpressionEda2r geneectodysplasin A2 receptorgeroprotectorстарениеэкспрессияген Eda2rрецептор эктодисплазина А2геропротектор1.Barbera, M. C., Guarrera, L., Re Cecconi, A. D., Cassanmagnago, G. A., Vallerga, A., Lunardi, M., ... & Bolis, M. (2025). Increased ectodysplasin-A2-receptor EDA2R is a ubiquitous hallmark of aging and mediates parainflammatory responses. Nature Communications, 16(1), 1898.2.Хафизова, А. З., Семина, И. И., Никитин, Д. О., & Мустафин, Р. И. (2025). Перспективы применения противодиабетического средства метформина как способ замедления биологического старения и возраст-ассоциированных заболеваний. Казанский медицинский журнал, 106(1), 105-116.3.Livak, K. J., & Schmittgen, T. D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. methods, 25(4), 402-408.