Молодежный инновационный вестникМолодежный инновационный вестник2415-7805Федеральное государственное бюджетное образовательное учреждение высшего образования "Воронежский государственный медицинский университет имени Н.Н. Бурденко" Министерства здравоохранения Российской Федерации10837UnclassifiedAge-related changes in microRNA let-7 expression in the dorsomedial and ventromedial hypothalamic nuclei of ratsPankrashevaLydia Georgievna<p>instructor at the <span class="EzKURWReUAB5oZgtQNkl" data-src-align="14:7">Department</span> of <span class="EzKURWReUAB5oZgtQNkl" data-src-align="22:10">Normal</span> <span class="EzKURWReUAB5oZgtQNkl" data-src-align="33:10">Physiology</span> and <span class="EzKURWReUAB5oZgtQNkl" data-src-align="46:10">Biophysics</span></p>white.corvus8@gmail.comhttps://orcid.org/0009-0006-9506-4374MasliukovPetr Mihailovich<p>doctor of Medical Sciences, Head of the Department of Normal Physiology and Biophysics</p>mpm@ysmu.ruhttps://orcid.org/0000-0002-6230-5024Yaroslavl State Medical University041120251421061091004202507082025Copyright © 2025, Pankrasheva L.G., Masliukov P.M.2025<p style="text-align: justify;">Aging is accompanied by complex changes in the regulation of metabolic processes, with hypothalamic structures, including its tuberal nuclei, playing a key role. Modern science pays particular attention to studying epigenetic mechanisms of aging, among which microRNAs occupy an important place. However, their role in age-related changes in the functional activity of hypothalamic neurons remains insufficiently studied.</p>
<p style="text-align: justify;">The aim. o investigate age-related changes in microRNA let-7 expression in the dorsomedial (DMH) and ventromedial (VMH) hypothalamic nuclei and evaluate their effect on neuronal activity.</p>
<p style="text-align: justify;">Materials and methods. The study was conducted on two age groups of male Wistar rats (young - 2 months and old - 24 months). Modern molecular biological methods were used: fluorescent in situ hybridization (FISH) and quantitative RT-PCR to assess let-7 expression, as well as electrophysiological methods for recording neuronal activity.</p>
<p style="text-align: justify;">Results. A significant (5-fold, p0.05) decrease in let-7 expression was found in the DMH of old animals compared to young ones, while no significant changes were detected in the VMH. At the same time, modulation of let-7 activity did not lead to significant changes in neuronal firing rate.</p>
<p style="text-align: justify;">Conclusions. The obtained data indicate an age-dependent decrease in let-7 expression in the DMH, which may be important for understanding the mechanisms of neuroendocrine regulation during aging. Further research in this direction may contribute to the development of new approaches to correcting age-related metabolic disorders.</p>microRNA let-7hypothalamusdorsomedial nucleusventromedial nucleusagingмикроРНК let-7гипоталамусдорсомедиальное ядровентромедиальное ядростарение[Masliukov PM, Nozdrachev AD. Hypothalamic regulatory mechanisms of aging. J Evo Biochem Physiol. 2021;57(3):473-491. https://doi.org/10.1134/s0022093021030030][Tran LT, Park S, Kim SK, Lee JS, Kim KW, Kwon O. Hypothalamic control of energy expenditure and thermogenesis. Exp Mol Med. 2022;54:358–369. https://doi.org/10.1038/s12276-022-00741-z][Bhusal A, Rahman MH, Suk K. Hypothalamic inflammation in metabolic disorders and aging. Cell. Mol. Life Sci. 2022; 79:32. https://doi.org/10.1007/s00018-021-04019-x][Chirshev E, Oberg KC, Ioffe YJ et al. Let-7 as biomarker, prognostic indicator, and therapy for precision medicine in cancer. Clin Trans Med. 2019;8:24. https://doi.org/10.1186/s40169-019-0240-y][Macvanin M, Obradovic M, Zafirovic S, Stanimirovic J, Isenovic ER. The Role of miRNAs in Metabolic Diseases. Current Medicinal Chemistry. 2023;30(17):1922-1944. https://doi.org/ 10.2174/0929867329666220801161536]