Молодежный инновационный вестник

2415-7805

Федеральное государственное бюджетное образовательное учреждение высшего образования "Воронежский государственный медицинский университет имени Н.Н. Бурденко" Министерства здравоохранения Российской Федерации

10795

Unclassified

The specifics of skin regeneration depending on the nature of damage (experimental study)

Panina

Polina Sergeevna

panina250305@mail.ruhttps://orcid.org/0009-0005-4700-9381Steinfeld

Alexandra Alexandrovna

a.shteynfeld@mail.ruhttps://orcid.org/0009-0008-2959-7521Buglak

Anna Olegovna

Senior Lecturer at the Department of Anatomy, Histology and Embryologysnej04ik@gmail.comhttps://orcid.org/0009-0005-0534-7921Ganina

Ekaterina Borisovna

PhD, Senior Lecturer at the Department of Anatomy, Histology and Embryologykatena-ganina@mail.ruhttps://orcid.org/0000-0002-8686-8526

Tver State Medical University

04112025

142105106

31032025

30072025

2025

<div> n this research paper, we studied the features of skin regeneration in rats (in particular, the rate) depending on the nature of the wound: aseptic, infected, thermal and chemical(acid and alkaline) burns.The experiment was conducted on 20 male outbred white rats for 45 days. During the study, external examination of animals, measurement of the area of skin wounds and photodocumentation of animals were carried out daily. After processing the obtained data and analyzing them, the results were entered into the table for further charting in order to track the dynamics of wound healing.The results showed that all wounds underwent stages of hemostasis, inflammation, proliferation, and remodeling.Differences in the rate and other features of regeneration were found depending on the wound etiology.Aseptic wounds healed faster than others 14 days; infected wounds-30 days, recovery was accompanied by the appearance of foci of inflammation over the entire surface of the rat body; acid burn-36 days; thermal and alkaline burns (41 days and 42 days, respectively) showed the slowest regeneration.The study showed a relationship between the healing rate and the nature of the wound, which emphasizes the need for a differentiated approach to the treatment of skin injuries. </div>

modelingregenerationratswound areaepithelializationskin

моделированиерегенерациякрысыплощадь раныэпителизациякожа

Introduction. The relevance of the study is due to the high prevalence of skin trauma of various etiologies. A variety of causes of skin damage causes specific changes in tissues, which affects the course of regenerative processes. Understanding the features of regeneration is a key factor in the development of new effective treatment methods, as well as in determining the strategy and tactics of treatment. The purpose of the work is to study the specifics of regeneration of skin damage resulting from modeling aseptic and infected wounds, thermal and chemical burns in an experiment on rats. Materials and methods of research. The study was conducted on male outbred white rats (n = 20) aged 8-9 months and with an average body weight of 300 grams. The animals were divided into five groups of 4 rats each. The following injuries were modeled on the back: aseptic wound, wound aggravated by infection (Staphylococcus aureus), thermal, chemical burn with acid and alkali. The animals were examined daily, the wounds were measured and photographed to monitor the healing process. The obtained data were processed and analyzed, the results were entered into a table for plotting graphs [1, 2]. Research results. During the healing process, all wounds went through four main stages of regeneration: hemostasis, inflammation, proliferation and remodeling. Complete epithelialization of the aseptic wound and alkali burn occurred on the 21st day, the infected wound - on the 30th day, the acid burn - on the 36th day, the thermal burn - on the 41st day. In rats exposed to chemical acid burns, the inflammation stage was especially active and prolonged. Infected wounds were accompanied by the appearance of foci of inflammation in previously undamaged areas. Conclusion. The healing process of the skin in all experimental animals went through four classical stages. However, the speed of their progression and nature could differ. The experiment proved the need for an individual approach to the treatment of skin wounds, despite the general trends of regeneration processes.

Донсков С. А. Техника безопасности при работе студентов в гистологической лаборатории в рамках учебного процесса / С. А. Донсков, Е. Б. Ганина, В. Г. Шестакова // «Актуальные проблемы безопасности жизнедеятельности и экологии»: материалы II Международной научно-практической конференции с научной школой для молодежи Минобр и науки РФ ФБУЗ ВО «Тверской государственный технический университет» (24-27 марта 2016 года). – Тверь, 2016. - С. 253-254.

Буглак А.О. Тезиографический метод исследования / А.О. Буглак, Шестакова В.Г., Е.Б. Ганина, В.Н. Захарова, В.В. Патрошкина. - Текст : непосредственный // Тверской медицинский журнал. 2023. - №5.