Молодежный инновационный вестникМолодежный инновационный вестник2415-7805Федеральное государственное бюджетное образовательное учреждение высшего образования "Воронежский государственный медицинский университет имени Н.Н. Бурденко" Министерства здравоохранения Российской Федерации10408Conference ProceedingsComparative analysis of heavy metals by their ability to increase membrane permeability of erythrocytesProtsenkoDiana Igorevna<p>2nd year student of the Faculty of Medicine</p>diana.kitty.04@yandex.ruhttps://orcid.org/0009-0006-1657-377XAntipovEvgeniy Valerievich<p>Candidate of Biological Sciences, Associate Professor of the Department of Natural Sciences</p>eugantipov@gmail.comPrivate institution educational organization of higher education Medical University "Reaviz"2504202514S155592502202524032025Copyright © 2025, Protsenko D.I., Antipov E.V.2025<p style="font-weight: 400;"><em>Heavy metals that enter the human body with drinking water or when inhaling air polluted by emissions contribute to damage to cell membranes, initiating processes of free radical lipid peroxidation, under the influence of which osmotic resistance can change. Objective: to evaluate the effect of barium, manganese and strontium salts on the osmotic resistance of erythrocytes. Materials and methods: the object of the study was a suspension of washed erythrocytes. The experimental samples containing 10 mM solutions of barium, manganese and strontium salts contained 0.01 ml of erythrocyte suspension, 5 ml of 0.5% (or 0.9%) sodium chloride solutions. After incubation (37 C, 30 min), the optical density of the solutions (540 nm) was measured on a PE-5400 UV spectrophotometer. The number of cells subjected to hemolysis was determined as a percentage of complete hemolysis. Results: it was found that in samples containing physiological sodium chloride solution, with the addition of 10 mM barium solution, the number of hemolyzed cells increased by 9.6 times, with manganese by 2.4 times, with strontium - by 8 times compared to the control. In samples with a hypotonic sodium chloride solution, the number of hemolyzed cells after 30 minutes of incubation reached 90% in samples with barium, 52% with manganese, 97% with strontium. Comparative analysis of the effect of heavy metals on the osmotic resistance of erythrocytes in vitro showed that barium salts have the greatest hemolytic effect, and manganese salts have the least. The data obtained indicate a damaging effect of heavy metal salts on erythrocytes, characterized by deterioration of the properties of the erythrocyte membrane due to the initiation of lipid peroxidation processes in it.</em></p>erythrocytesfree radical oxidationheavy metalsosmotic resistanceэритроцитысвободнорадикальное окислениетяжелые металлыосмотическая резистентность1.Евстратова О.Р., Харитонова А.С., Лущик М.В. Роль процессов свободнорадикального окисления в развитии патологий // Международный студенческий научный вестник. 2016. № 4-2.2.Гривенникова В. Г., Виноградов А.Д. Генерация активных форм кислорода митохондриями // Успехи биологической химии. 2013. Т.53. С. 245-296.3.Аралбаева А.Н., Маматаева А.Т., Утегалиева Р.С., Мурзахметова М.К. Сравнительное изучение влияния тяжелых металлов на резистентность мембран эритроцитов в условиях in vitro // Experimental Biology. №3 (80). 2019. С. 182-191.:4.Кленова Н.А., Кленов Р.О. Строение, метаболизм и функциональная активность эритроцитов человека в норме и патологии. – Самара: Издательство «Самарский университет», 2009. – 116 с.5.Муравьев А.В. Вне- и внутриклеточные механизмы изменения агрегации эритроцитов // Физиология человека. 2005. Т. 31. №4. С. 108-112.6.Антипов Е. В. Медицинская экология с основами радиобиологии: учебное пособие. – Самара: Самарский медицинский институт «РЕАВИЗ», 2016. – 230 с.