Молодежный инновационный вестникМолодежный инновационный вестник2415-7805Федеральное государственное бюджетное образовательное учреждение высшего образования "Воронежский государственный медицинский университет имени Н.Н. Бурденко" Министерства здравоохранения Российской Федерации10365Conference ProceedingsComplex micromorphometric parameters of hepatocytes of Wistar rats at the age of three monthsAnurkinaAnna I.anyaaai1925@gmail.comhttps://orcid.org/0009-0003-0011-1114BorisenkoMarina B.marina-borisenko-2000@mail.ruhttps://orcid.org/0000-0002-2684-2017KozlovaMariya A.kma-morph@mail.ruhttps://orcid.org/0000-0001-6251-2560AreshidzeDavid A.labcelpat@mail.ruhttps://orcid.org/0000-0003-3006-62812504202514S12222252402202524022025Copyright © 2025, Anurkina A.I., Borisenko M.B., Kozlova M.A., Areshidze D.A.2025<p>At the stage of preclinical research of drugs, tests are carried out on laboratory animals. For scientific purposes, Wistar rats are most often used due to their small mass, relative simplicity of housing and breeding. To obtain the most reliable and correctly interpreted results, it is important to create bases of health-associated reference intervals (RI) of biometric parameters of laboratory animals depending on the sex and age of animals, season of research and reaction to external influences. Objective. To study micromorphometric indices of hepatocytes of male and female Wistar rats in normals at the age of 3 months. Materials and methods. 80 Wistar rats were kept in standard conditions in accordance with GOST R 53434-2009 for 3 weeks with subsequent euthanasia and evisceration of the liver, as well as determination of its absolute and relative weight Morphometric analysis was carried out on liver micropreparations stained with hematoxylin and eosin. Micromorphometry was performed only on interphase hepatocytes without signs of pathological changes. Results. We obtained data of micromorphometric parameters of hepatocytes characteristic for male and female Wistar rats at the age of 3 months. As a result, a clear gender dimorphism of these values is observed.</p>Reference intervalsRIhepatocytesWistar ratsmicromorphometric parameterspreclinical researchРеференсные интервалы (РИ)гепатоцитыкрысы Вистармикроморфометрические показателидоклинические исследования1.Schneider CA, Rasband WS, Eliceiri KW. NIH Image to ImageJ: 25 years of image analysis. Nat Methods. 2012;9(7):671-675. doi:10.1038/nmeth.20892.Broeke J, Mateos Pérez JM, Pascau J. Image processing with ImageJ: extract and analyze data from complex images with ImageJ, the world’s leading image processing tool. Second edition. Birmingham Mumbai: Packt Publishing open source; 2015. 231 p.3.Smitha T, Sharada P, Girish H. Morphometry of the basal cell layer of oral leukoplakia and oral squamous cell carcinoma using computer-aided image analysis. J Oral Maxillofac Pathol. 2011;15(1):26-33. doi:10.4103/0973-029X.800344.Автандилов Г.Г. Основы количественной патологической анатомии: Учебное пособие.-М, 2002 (рец. ЛВ Кактурский) // Архив патологии. – 2004. – Т. 66. – №. 3. – С. 61-61.5.Rando G, Wahli W. Sex differences in nuclear receptor-regulated liver metabolic pathways. Biochim Biophys Acta. 2011;1812(8):964-973. doi:10.1016/j.bbadis.2010.12.0236.Zheng D, Wang X, Antonson P, et al. Genomics of sex hormone receptor signaling in hepatic sexual dimorphism. Mol Cell Endocrinol. 2018;471:33-41. doi:10.1016/j.mce.2017.05.0257.Kitagawa T, Yokoyama Y, Kokuryo T, et al. Estrogen promotes hepatic regeneration via activating serotonin signal. Shock. 2009;31(6):615-620. doi:10.1097/SHK.0b013e31818ec195