BIOCRYSTALLOMICS AS A METHOD OF PREDICTING THE COURSE OF REPAIR OF BURN WOUNDS OF THE SKIN
- Authors: Buglak A.O.1, Shestakova V.G.1, Ganina E.B.1
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Affiliations:
- Tver State Medical University
- Issue: Vol 12, No 1 (2023): Материалы XVII Международной научно-практической конференции молодых ученых-медиков
- Pages: 24-25
- Section: СОВА (Архив)
- URL: https://new.vestnik-surgery.com/index.php/2415-7805/article/view/8785
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Abstract
In pathological processes, there is a change in the chemical composition of tissues and body fluids, which can fix it by crystallizing the object under study. The change in the chemical composition of plasma is important in the dynamics of the skin regeneration process. The aim of the study was a qualitative and quantitative assessment of the plasma crystallization process and the determination of the dependence of the crystallomorphological pattern on the timing of repair and the change of phases of healing of burn wounds. The study was conducted on 12 white mongrel rats kept in the same vivarium conditions, with a standard maintenance regime. Thermal burn on the dorsal surface of the body was simulated in rats. Blood plasma was taken for the study on the 7th, 14th, 21st days of the experiment. The plasma obtained from experimental animals was compared with the plasma of an intact rat. A 2% solution of ninhydrin was used as a diagnostic solution. Stereometric processing of the results consisted in calculating the density of crystallization centers. As a result, crystals of different sizes and shapes were obtained. According to the results of the study, it can be concluded that plasma crystallomorphology can be used as one of the additional methods of investigation and diagnosis of regeneration processes of burn wounds.
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Relevance. When studying the features of plasma crystallization, it was found that it can serve as an objective indicator of the functional state of the organism.
The purpose of the study. Qualitative and quantitative assessment of the plasma crystallization process and determination of the dependence of the crystallomorphological pattern on the timing of repair and the change of phases of healing of burn wounds.
Materials and methods. The study was conducted on 12 male mongrel white rats. All experimental studies were conducted in compliance with the rules and regulations for working with experimental animals [1].Thermal burns with a total area of 225 mm2 were applied to the animals on the pre-prepared dorsal surface of the body using a special soldering iron. Plasma was taken from animals on the 7th, 14th, 21st days of the experiment.For diagnosis, 2 ml of plasma was taken, to which a two percent alcohol solution of ninhydrin (10 ml) was added. The mixture was poured into a Petri dish and left for 12-14 hours to crystallize. Then the crystals were examined under a light microscope and photographed.The crystallized plasma of an intact rat was used as a reference.Stereometric processing of the results consisted in calculating the density of crystallization centers. Nine visual fields were viewed on the measured surface in two mutually perpendicular directions and the number of crystallization centers within each field was calculated [2].
Results. When the alcohol solution of ninhydrin is crystallized without additives, large crystals are formed in the form of regular spherulites. The shape and size of the crystals changed when rat plasma was added to the alcohol solution of ninhydrin at different stages of repair.
Conclusions. Plasma crystallomorphology can be used as one of the additional methods of investigation and diagnosis of regeneration processes of burn wounds.
About the authors
Anna Olegovna Buglak
Tver State Medical University
Email: snejo4ik@gmail.com
ORCID iD: 0009-0005-0534-7921
SPIN-code: 8390-3720
Russian Federation, 1, Ivan Sedykh str., Tver, 170100, Russia
Valeria Gennadievna Shestakova
Tver State Medical University
Email: shestvg@mail.ru
ORCID iD: 0000-0003-1136-7396
Russian Federation, 1, Ivan Sedykh str., Tver, 170100, Russia
Ekaterina Borisovna Ganina
Tver State Medical University
Author for correspondence.
Email: katena-ganina@mail.ru
ORCID iD: 0000-0002-8686-8526
SPIN-code: 1217-7179
Russian Federation, 1, Ivan Sedykh str., Tver, 170100, Russia
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