Morphological aspects of prevention of postoperative hepatic insufficiency with cyanocobalamin solution


Cite item

Abstract

Goal. Improving the results of prevention of postoperative hepatic insufficiency by activating the reparative potential of the liver with cyanocobalamin administration.

Materials and methods. The study was performed on 36 Wistar rats. In 3 experimental groups, resection was performed in the amount of 70% of the initial liver mass: in the 1st - prevention of postoperative liver failure was not carried out, in the 2nd - after resection, 0.9% NaCl solution was injected into the preserved liver lobes, in the 3rd - cyanocobalamin. Liver resection was not performed in the 1st control group. The result was evaluated 2 weeks after surgery. The MS Excel package was used for statistical processing.

Results. Histological and immunohistochemical studies 2 weeks after surgery showed the best results in the 3rd experimental group: the number of binucleated hepatocytes was 19.31±3.68%, ki67-positive hepatocyte nuclei 6.22± 0.54%. When studying liver sections stained with silver impregnation, the number of reticular fibers in the 3rd experimental group was more than 90% of the indicators of the 1st control group, in the 1st and 2nd experimental groups – less than 80%.

Conclusion. The proposed method of activating the reparative potential of the liver by the introduction of cyanocobalamin allows to increase the proliferative activity of hepatocytes, contributes to an increase in the number of cells entering the mitotic cycle, ensures the restoration of the structural organization of the liver, its anatomical and functional integrity.

Full Text

Relevance. Liver resection in most cases remains one of the optimal ways to achieve an increase in life expectancy and cure of patients [1-5]. As a rule, operations with the removal of three or more segments are used for tumor and parasitic diseases, which in most cases is the cause of severe complications and post-resection liver failure [2-7].

Goal. Improving the results of prevention of postoperative hepatic insufficiency by activating the reparative potential of the liver with cyanocobalamin administration.

Materials and methods. The study was performed on 36 Wistar rats. In 3 experimental groups, resection was performed in the amount of 70% of the initial liver mass: in the 1st - prevention of postoperative liver failure was not carried out, in the 2nd - after resection, 0.9% NaCl solution was injected into the preserved liver lobes, in the 3rd - cyanocobalamin. Liver resection was not performed in the 1st control group. The result was evaluated 2 weeks after surgery. The MS Excel package was used for statistical processing.

Results. In histological and immunohistochemical studies 2 weeks after surgery, the best results were observed in the 3rd experimental group: the number of binucleated hepatocytes was 19.31±3.68%, ki67-positive hepatocyte nuclei 6.22± 0.54%, which exceeded the indicators of the 1st and 2nd experimental groups by 1.5 and 3 times, respectively. When studying liver sections stained with silver impregnation, the number of reticular fibers in the 3rd experimental group was more than 90% of the indicators of the 1st control group, in the 1st and 2nd experimental groups – less than 80%.

Conclusion. The proposed method of activating the reparative potential of the liver by the introduction of cyanocobalamin allows to increase the proliferative activity of hepatocytes, contributes to an increase in the number of cells entering the mitotic cycle, ensures the restoration of the structural organization of the liver, its anatomical and functional integrity.

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About the authors

Victoria Viktorovna Shishkina

Voronezh State Medical University named after N.N. Burdenko

Email: 4128069@gmail.com

Director of the Research Institute of Experimental Biology and Medicine, Candidate of Medical Sciences, Associate Professor

Russian Federation, 394036, Russia, Voronezh, 10 Studentskaya str.

Anastasia Yurievna Laptiyova

Voronezh State Medical University named after N.N. Burdenko

Author for correspondence.
Email: laptievaa@mail.ru
ORCID iD: 0000-0002-3307-1425
SPIN-code: 7626-9016

Assistant

Russian Federation, 394036, Russia, Voronezh, 10 Studentskaya str.

Anton Petrovich Ostroushko

Voronezh State Medical University named after N.N. Burdenko

Email: oap@vrngmu.com
ORCID iD: 0000-0003-3656-5954
SPIN-code: 9811-2385

Candidate of Medical Sciences, Associate Professor

Russian Federation, 394036, Russia, Voronezh, 10 Studentskaya str.

Maria Alexandrovna Yashkova

Voronezh State Medical University named after N.N. Burdenko

Email: YshkovaMasha@yandex.ru

Student, Voronezh State Medical University named after N.N. Burdenko

Russian Federation, 394036, Russia, Voronezh, 10 Studentskaya str.

References

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  7. Андреев А.А., Шишкина В.В., Лаптиева А.Ю., Глухов А.А., Остроушко А.П.. Особенности пострезекционной регенерации гепатоцитов под влиянием внутрипеченочного введения цианокобаламина. Журнал анатомии и гистопатологии. 2021. Т. 10. № 3. С. 27-34.

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