Biochemical aspects of prevention of chronic liver failure after liver resection
- Authors: Laptiyova A.Y.1, Antakova L.N.1, Ostroushko A.P.1
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Affiliations:
- Voronezh State Medical University named after N.N. Burdenko
- Issue: Vol 11, No 2 (2022): Materials of the XV International Surgical Forum "INNOVATIVE TECHNOLOGIES IN SURGERY"
- Pages: 64-66
- Section: Хирургические дисциплины
- URL: https://new.vestnik-surgery.com/index.php/2415-7805/article/view/7652
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Abstract
Goal. Improving the results of the prevention of chronic liver failure by intraoperative administration of cyanocobalamin in an experiment.
Materials and methods. The experiments were performed on 96 Wistar rats. All animals underwent liver resection according to the method of G. Higgins and R.Anderson. In the 1st control group, prevention of liver failure was not carried out, in the 2nd control group, 0.9% NaCl was intrahepally administered; in the 1st experimental group, cyanocobalamin; in the 2nd experimental group, cyanocobalamin was administered intraperitoneally. On the 14th day, blood was taken for biochemical examination. Statistical processing was performed using the Statistica 6.0 program.
Results. On the 14th day of the study, normalization of cytolysis syndrome indicators was observed in the 1st experimental group in 97.5 ± 3.1% of animals; in the 2nd experimental group - in 71.0 ± 3.1%, in the 1st control group - in 68.2 ± 4.1%, in the 2nd control group – in 84.6± 2.6% of animals. Indicators of hepatic cell insufficiency syndrome normalized in 100% of animals in the 1st experimental group, 88.4±4.1% - in the 1st control group, 84.3±2.2% - in the 2nd control group, 91.1±2.4% – in the 2nd experimental group. Indicators of cholestatic syndrome were normalized only in the 1st experimental group in 68.4 ± 5.2% of animals.
Conclusion. Intraoperative administration of cyanocobalamin during liver resection significantly contributes to the relief of cytolysis, cholestasis and hepatic cell insufficiency syndromes.
Keywords
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Relevance. Liver surgeries continue to be traumatic high-risk interventions due to the formation of a powerful stress response of the body induced by tissue damage, the risk of severe intra- and postoperative complications, as well as high mortality in patients even without structural and functional liver disorders [1-5]. To date, postresection hepatic insufficiency (PPN) develops in 32-60% of cases, which complicates the course of the postoperative period and rehabilitation of patients [2-6].
Goal. Improving the results of the prevention of chronic liver failure by intraoperative administration of cyanocobalamin in an experiment.
Materials and methods. The experiments were performed on 96 Wistar rats. All animals underwent liver resection according to the method of G. Higgins and R.Anderson. In the 1st control group, prevention of liver failure was not carried out, in the 2nd control group, 0.9% NaCl was intrahepally administered; in the 1st experimental group, cyanocobalamin; in the 2nd experimental group, cyanocobalamin was administered intraperitoneally. On the 14th day, blood was taken for biochemical examination. Statistical processing was performed using the Statistica 6.0 program.
Results. On the 14th day of the study, normalization of cytolysis syndrome indicators was observed in the 1st experimental group in 97.5 ± 3.1% of animals; in the 2nd experimental group - in 71.0 ± 3.1%, in the 1st control group - in 68.2 ± 4.1%, in the 2nd control group – in 84.6± 2.6% of animals. Indicators of hepatic cell insufficiency syndrome normalized in 100% of animals in the 1st experimental group, 88.4±4.1% - in the 1st control group, 84.3±2.2% - in the 2nd control group, 91.1±2.4% – in the 2nd experimental group. Indicators of cholestatic syndrome were normalized only in the 1st experimental group in 68.4 ± 5.2% of animals.
Conclusion. Intraoperative administration of cyanocobalamin during liver resection significantly contributes to the relief of cytolysis, cholestasis and hepatic cell insufficiency syndromes. The proposed method of prevention provides normalization of metabolic processes of the liver in the early postoperative period, which excludes the possibility of developing chronic liver failure in the long term after surgery.
About the authors
Anastasia Yurievna Laptiyova
Voronezh State Medical University named after N.N. Burdenko
Email: laptievaa@mail.ru
ORCID iD: 0000-0002-3307-1425
SPIN-code: 7626-9016
Assistant
Russian Federation, 394036, Russia, Voronezh, 10 Studentskaya str.Lyubov Nikolaevna Antakova
Voronezh State Medical University named after N.N. Burdenko
Author for correspondence.
Email: tsvn@bk.ru
ORCID iD: 0000-0002-1825-0097
SPIN-code: 3936-3381
Head of the Laboratory of Postgenomic Research, Candidate of Biological Sciences, Senior Researcher
Russian Federation, 394036, Russia, Voronezh, 10 Studentskaya str.Anton Petrovich Ostroushko
Voronezh State Medical University named after N.N. Burdenko
Email: oap@vrngmu.com
ORCID iD: 0000-0003-3656-5954
SPIN-code: 9811-2385
Candidate of Medical Sciences, Associate Professor
Russian Federation, 394036, Russia, Voronezh, 10 Studentskaya str.References
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