WOUND-HEALING ACTIVITY OF THE EXPERIMENTAL THYMOGEN ANALOGUE


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Abstract

The aim is to investigate the possibility of reparative effects in a new thymogen analog modified with D-Ala from the C-terminus of the molecule.
Materials and methods. In 60 Wistar rats, skin damage was modeled, after which thymogen and its analogue modified with D-Ala from the C-terminus of the peptide were injected intraperitoneally at equimolar concentrations. A histological examination of sections of skin wounds was carried out.
Results. An increase in lymphocytes and fibroblasts was found in the groups receiving peptides. The action of the analog was more pronounced in relation to fibroblasts (p<0.05).
Conclusions. Thus, the reparative activity of the experimental analogue of thymogen turned out to be higher than that of the original preparation.

 

Full Text

Relevance. One of the most important medical and social problems of the countries of the modern world is traumatism, which is common among all segments of the population. The effectiveness of peptide preparations has been proven, which made it possible to use them in clinical practice [1, 2]. The rate of reparative regeneration depends on the functional activity of the immune system; therefore, the pharmacological preparation thymogen, consisting of L-configurations of glutamic acid and tryptophan and having an immunomodulatory effect, was chosen for the experiment. We assumed that the chemical modification of peptides can lead to an increase in the severity of their action.

The aim of the study was to investigate the possibility of reparative effects in a new thymogen analogue modified with D-Ala from the C-terminus of the molecule.

Material and methods. The study was conducted on 60 male Wistar rats, which were subjected to skin wounds at the withers under chloral hydrate anesthesia according to the generally accepted method [3]. Animals received intraperitoneally in equimolar doses for 10 days thymogen (1 μg/kg) and its new analog (1.2 μg/kg) synthesized at the Research Institute of Chemistry of St. Petersburg State University. The control group received isotonic sodium chloride solution. Histological examination of skin wounds was carried out. The tissues were fixed in 10% neutral formalin solution, after which they were embedded in paraffin and sections were prepared, stained with hematoxylin-eosin [1, 3]. The percentage ratio of lymphocytes and fibroblasts (per 100 cells) was determined in the papillary layer of the skin using a Leica CME light microscope. To determine the significance of the difference between the groups, the boundaries of the divergence of confidence intervals and the nonparametric Mann-Whitney test were calculated.

Results. A comparable increase in the number of lymphocytes was found both with the introduction of the original drug (25.4±1.6%) and with the introduction of its experimental analogue (24.6±1.4%), which may be associated with the immunostimulatory effect of peptides under conditions immune disorders resulting from skin damage. Peptides also increased the number of fibroblasts, but for thymogen (29.6±1.7%) the difference compared to the control group (29.3±1.2%) was not significant. At the same time, the analog turned out to be more effective (34.7±1.5%), which makes it possible to judge its wound healing effect. The mechanism of these phenomena is probably associated with the activation of reduced immune function.

Conclusions. Thus, the reparative activity of the experimental analogue of thymogen was higher than that of the original preparation by 5.1%.

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About the authors

Angelina Mikhailovna Smakhtina

Kursk State Medical University

Author for correspondence.
Email: smaxtina2012@yandex.ru
Russian Federation

Anna Alexandrovna Chulanova

Kursk State Medical University

Email: chulanofs@mail.ru
Russian Federation

Vadim Ruslanovich Rukavitsyn

Kursk State Medical University

Email: vadimrukavitsyn@yandex.ru
Russian Federation

Stanislav Ruslanovich Gunov

Kursk State Medical University

Email: gunov99@yandex.ru
Russian Federation

References

  1. Синергичное иммунотропное и репаративное действие тимогена, даларгина и регуляторного пептида Gly-His-Lys при экспериментальной травме кожи / В.Ю. Чердаков, М.Ю. Смахтин, Г.М. Дубровин, И.И. Бобынцев / Курский научно-практический вестник «Человек и его здоровье». – 2013. – № 1. – С. 38–42.
  2. Влияние пептида Gly-His-Lys и его аналогов на болевую чувствительность у мышей / И.И. Бобынцев, О.И. Чернышева, М.Е. Долгинцев и др. // Экспериментальная и клиническая фармакология. – 2015. – № 78(1). – С. 13–15.
  3. Влияние аминокислот, составляющих пептид глицил-гистидил-лизин, на регенерацию кожных ран и функцию нейтрофилов / А.А. Курцева, М.Ю. Смахтин, А.В. Иванов, А.В. Беседин // Курский научно-практический вестник «Человек и его здоровье». – 2008. – № 3. – С. 5–10.

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