• Authors: Rjadnova V.1
  • Affiliations:
    1. Курский государственный медицинский университет
  • Pages: 22-23
  • Section: Результаты фундаментальных и прикладных исследований
  • URL:

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The article discusses the effect of the systemic disinfectant thiram on the quantitative and qualitative composition of the obligate microflora of the large intestine of experimental animals.

Full Text

Relevance. In agro-industrial production, pesticides are an important means of protecting plants and increasing crop yields. A wide range of pesticides are used to control diseases, pests, and preserve crops [1]. The widespread use of pesticides and other persistent organic pollutants results in water, soil, air and biota pollution. Getting into the ecosystem, xenobiotics pose a threat to humans, interfere with the normal functioning of many body systems, and especially the digestive system [2, 3]. Purpose of the study. To study the state of the obligate microflora of the large intestine of experimental animals with subchronic intoxication with thiram fungicide. Materials and methods. Mature Wistar rats were chosen for the experiment. According to the method of L.I. Kafarskaya and V.M. Korshunov, the parietal microflora of the colon was studied (qualitative and quantitative). Microorganisms were identified using a Maldi Biotyper Microflex mass spectrometer. Results. The experimental animals were divided into 5 groups of 30 males each. The first, control group and four groups of animals received tiram with standard food for 7, 14, 21 and 28 days. The following representatives of the obligate microflora were registered in the control group: Lactobacillus (6.32±0.66), Bifidobacterium (8.95±1.29) and E. coli with normal enzymatic activity (4.75±0.60). On the 7th day of thiram use, there was a decrease in the qualitative and quantitative representatives of the obligate microflora: lactobacilli (3.89±0.41), bifidobacteria (5.43±0.54), Escherichia with normal enzymatic activity (4.05±0.49) . On the 14th and 21st days of thiram use, a decrease in obligate microorganisms was also observed. More pronounced changes were observed on the 28th day, bifidobacteria decreased from lg 8.95±1.29 to lg 3.00±0.27; lactobacilli - from lg 6.32±0.66 to lg 2.50±0.25; Escherichia with normal enzymatic activity - c lg 4.75±0.60 to lg 2.18±0.18. Conclusions. The use of the fungicide thiram in experimental animals led to a qualitative and quantitative decrease in all representatives of the obligate microflora of the large intestine. The number of lactobacilli on the 28th day decreased by 2.5 times, bifidobacteria by 3 times and Escherichia with normal enzymatic activity by 2.2 times compared with the group of intact animals. The data obtained show that the studied fungicidal preparation affects the microbiocenosis during the entire period of intoxication, and the duration of its use can lead to a significant decrease in the quantitative index of the obligate representatives of the microbiocenosis.


About the authors

Vera Rjadnova

Курский государственный медицинский университет

Author for correspondence.
Russian Federation


  1. Hassaan M.A., Nemr A. El. Pesticides pollution: Classifications, human health impact, extraction and treatment techniques. The Egyptian Journal of Aquatic Research. 2020; Vol. 46(3): 207-220p.
  2. Королёв В.А., Иванов В.П., Шорманов В.К., Ким А.В., Юшин В.В., Кирищева Н.Е., Никитина Е.С. Относительный экологический риск формирования детской патологии в условиях интенсивного применения фунгицида ТМТД // Курский научно-практический вестник "Человек и его здоровье", 2012, № 1. С.25-28.)
  3. Warra A.A., Prasad M.N.V. African perspective of chemical usage in agriculture and horticulture-their impact on human health and environment. Agrochemicals Detection, Treatment and Remediation Pesticides and Chemical Fertilizers. 2020, сhapter 16.401-436р.

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