The effect of rubidium chloride on the antioxidant defense system in the relief of experimental osteomyelitis
- Authors: Menshikova S.S.1, Sakovich N.V.1, Zolotukhin V.1
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Affiliations:
- Voronezh State Medical University named after N.N. Burdenko
- Issue: Vol 9 (2020): Материалы XVI Международной Бурденковской научной конференции 23-25 апреля 2020 года
- Pages: 413-414
- Section: Хирургические дисциплины
- URL: https://new.vestnik-surgery.com/index.php/2415-7805/article/view/6092
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Abstract
Osteomyelitis is a purulent-necrotic process affecting all layers of the topographic and anatomical affected area, occurring in 3-10% of cases in purulent surgery and recurrent after surgical treatment in 10-40% of patients. Until now, osteomyelitis is a serious socio-economic problem with a large percentage of population disability.
The goal is to develop a new method of complex treatment of osteomyelitis using rubidium chloride and to study the effectiveness of its influence on the parameters of lipid peroxidation and the antioxidant system.
Materials and methods. An experimental study was carried out in 5 groups (2 control and 3 experimental) on 175 white laboratory rats weighing 300 ± 20 g. Osteomyelitis of the lower third of the thigh was simulated. In the course of the study, an assessment was made of the objective state of animals and indicators of biochemical blood analysis, namely, the content of lipid peroxidation products (LPO) - DNPH, and the antioxidant system (AOS) - LPO SOD (DNPH) and AOS (SOD) against the background of the treatment. The results were assessed on the 7th, 14th and 28th days. Statistical analysis of the data obtained was carried out using Microsoft Excel software.
Research results. On the 7th day, an increase in the level of DPNH was noted in all experimental groups, which indicates a high activity of oxidative stress during the period of illness. The indicators of antioxidant protection (SOD) on the 7th day were significantly reduced in all groups, except for the 3rd experimental group (0.376 ± 0.03 units / ml; 0.689 ± 0.07; 0.567 ± 0.04; 0.735 ± 0.03; 0.819 ± 0.02, respectively), compared with the values of intact animals (0.741 ± 0.05), which indicates profound disturbances in the body of animals, and the importance of the experimental treatment in maintaining antioxidant protection. In the 3rd experimental group, the SOD level on the 28th day remains at a level (0.687 ± 0.02), close to the values of intact animals, which indicates compensation of oxidative processes, and balanced treatment, which contributes to the preservation of the activity of antioxidant defense in animals 3 -th experimental group, while the indicators in other groups are below the specified indicators.
Conclusions. The study suggests that the proposed method using a combination of rubidium chloride, amikacin and hydroxyapatite has a positive effect on the dynamics of the antioxidant system indicators, which, in turn, reduces the activity of lipid peroxidation processes.
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RELEVANCE. Osteomyelitis is a purulent-necrotic process affecting all layers of the topographic-anatomical affected area, occurring in 3-10% of cases in purulent surgery and recurrent after surgical treatment in 10-40% of patients [1, 2, 3]. Until now, osteomyelitis is a serious socio-economic problem with a large percentage of population disability [2, 3, 4, 5]. With this pathology, a comprehensive personalized treatment is indicated, which is selected taking into account the causative agent of the disease and its sensitivity to antibiotic therapy, the neglect of the process and the comorbidity of the patient.
PURPOSE OF THE STUDY. To develop a new method of complex treatment of osteomyelitis using rubidium chloride and to study the effectiveness of its influence on the parameters of lipid peroxidation and the antioxidant system.
METHODS. The experimental study was carried out at the Research Institute of Experimental Biology and Medicine in 5 groups (2 control and 3 experimental) on 175 white laboratory rats. During the experiments, sexually mature males weighing 300 ± 20 g without concomitant pathology were used. Modeling of osteomyelitis of the lower third of the thigh was carried out by creating a bone cavity and introducing into it a strain of Staphylococcus aureus (the choice of the pathogen is due to its occurrence in 60% of cases during bacteriological examination of the discharge of purulent wounds) [5]. Anesthesia was performed using Zoletil-100 at a dosage of 8 μg / kg. Animals of the 1st control group did not receive treatment; in the 2nd control group, debridement of the surgical wound was performed, which was also performed on animals in all experimental groups. In the 1st experimental group, additionally rubidium chloride powder was introduced into the bone cavity before filling it. In the 2nd experimental group, the treatment was performed similar to the 1st experimental group, but with the addition of powder (15 mg / kg of animal body weight). In the 3rd experimental group, the cavity was filled with powder of rubidium chloride, amikacin and calcium hydroxyapatite.
In the course of the study, the objective state of animals and indicators of biochemical blood analysis were assessed, namely, the content of lipid peroxidation products (LPO) - DNPH, and the antioxidant system (AOS) - LPO SOD (DNPH) and AOS (SOD). The results were assessed on the 7th, 14th and 28th days. Statistical analysis of the data obtained was carried out using Microsoft Excel software. Statistical data were processed using the Student's t-test with the calculation of the mean, standard deviation, differences were considered significant at p <0.05.
RESULTS. On the 7th day, in all experimental groups, there was an increase in the level of DPNH from the norm (N - 39.32 ± 2.36 optical density units / ml of serum): in the 1st control and 1st experimental indicators, they practically exceeded the norm 3 times, in other groups less than one and a half times. Such a significant increase in the level of DPNH indicates a high activity of oxidative stress during the period of illness.
On the 14th day in the control groups, there was a slight decrease in the level of DNFG (1st control - 79.52 ± 2.11; 2nd control - 67.84 ± 2.26) and a more pronounced drop in the experimental groups (1- I experienced - 72.05 ± 2.35; 2nd experienced - 58.63 ± 2.88; 3rd experienced - 54.13 ± 2.17), which indicates a more pronounced positive effect of combination therapy.
On the 28th day, there is also a slight decrease in the level of DNFG in both control groups and the experimental one: 1st control - 78.02 ± 2.15; 2nd control - 58.11 ± 2.72; 1st experimental - 64.94 ± 2.72, which indicates the continued high activity of lipid peroxidation. While in the 2nd experimental group the DNFH index approached the norm (44.58 ± 2.13), and in the 3rd experimental group it almost reached the normal level (44.24 ± 2.44).
The indicators of antioxidant protection (SOD) on the 7th day were significantly reduced in all groups, except for the 3rd experimental group (0.376 ± 0.03 units / ml; 0.689 ± 0.07; 0.567 ± 0.04; 0.735 ± 0.03; 0.819 ± 0.02, respectively), compared with the values of intact animals (0.741 ± 0.05), which indicates profound disturbances in the animals' organism. SOD indicators in the 3rd experimental group were within normal limits.
On the 14th day, SOD indicators slightly improved in all groups.
On the 28th day, there is also a decrease in the level of SOD in both control groups and the 1st experimental group: 1st control - 0.367 ± 0.04; 2nd control - 0.498 ± 0.02; 1st experimental - 0.412 ± 0.04, which indicates insufficient support of antioxidant protection in animals. In the 3rd experimental group, the SOD level remains at the level (0.687 ± 0.02), close to the values of intact animals, which indicates the compensation of oxidative processes, and balanced treatment, which contributes to the preservation of the activity of antioxidant defense in animals of the 3rd experimental group.
DISCUSSION. In the treatment of chronic osteomyelitis, an important role is played by an integrated approach to treatment, the components of which are a systemic effect on the body (antibacterial and anti-inflammatory therapy) and a local effect on the lesion. The drugs used in the local treatment of chronic osteomyelitis should have the following properties - antiseptic, analgesic, osteoconductive, osteoinductive, anti-inflammatory, antioxidant, hemostatic [6]. The proposed experimental method, consisting of such active components as rubidium chloride, hydroxyapatite and amikacin, has osteoconductive, antibacterial and immunostimulating effects [7].
Another important aspect in the treatment of inflammatory processes is the state of the antioxidant system, the effect of experimental treatment on which we were able to trace during the study.
CONCLUSION. The study suggests that the proposed method using a combination of rubidium chloride, amikacin and hydroxyapatite has a positive effect on the dynamics of antioxidant protection indicators, which, in turn, reduces the activity of lipid peroxidation processes and tissue damage.
About the authors
Svetlana S. Menshikova
Voronezh State Medical University named after N.N. Burdenko
Author for correspondence.
Email: MenshikovaSS@yandex.ru
ORCID iD: 0000-0002-4706-0276
SPIN-code: 4299-9889
Russian Federation, 394036, Russia, Voronezh, st. Student, 10
Nikita V. Sakovich
Voronezh State Medical University named after N.N. Burdenko
Email: dr.kit@mail.ru
ORCID iD: 0000-0002-7812-3807
SPIN-code: 9380-8400
Russian Federation, 394036, Russia, Voronezh, st. Student, 10
Vladimir Zolotukhin
Voronezh State Medical University named after N.N. Burdenko
Email: vladimir.zolotuxin@gmail.com
ORCID iD: 0000-0002-8513-3966
Russian Federation, 394036, Russia, Voronezh, st. Student, 10
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