Experimental study of the features of skin regeneration after an alkali burn
- Authors: Khasanova D.J.1, Osipova E.A.1, Ganina E.B.1, Buglak A.O.1
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Affiliations:
- Tver State Medical University
- Issue: Vol 14, No 2 (2025): Сова-2025. Материалы XIX Международной научно-практической конференции молодых ученых-медиков СОВА-2025
- Pages: 155-156
- Section: СОВА
- URL: https://new.vestnik-surgery.com/index.php/2415-7805/article/view/10870
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Abstract
The article is devoted tomodeling of an alkaline burn on intact rat skin in an experiment.The aim of the study is to model andstudy the effect of alkali on the skin and the nature of burn wound healing.The methodological framework includesapplying a chemical substance, namely alkali, to the dorsal surface of the rat body, further taking biopsies of healing wounds with adjacent areas of intact skin, and preparing paraffin blocks for subsequent preparation of skin sections. The main results of the study were obtained by analyzing histological preparations that show a change in the regeneration phases from hemostasis to remodeling of the liver.In the experiment, blood was alsodrawn from the femoral vein for a thesiographic study. The data of the thesiographic method allowed us to reveal the evolution of the crystal shape from hemispherolites to spherulites, in which a progressive increase in the number of rays was observed at the stage of remodeling. Thus, during the experiment, skin regeneration was observed, during which three phases can be distinguished: inflammation of the skin, cell proliferation and their maturation. The burn sites and healing time of the skin surface were also studied in detail, with further assessment of the wound healing process.
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Introduction. Chemical burns are a serious medical problem. Every year, 18,000 people die from burns caused by improper handling of chemicals or industrial accidents.The study of the effects of alkali on the skin of rats will allow us to study in detail the processes at the cellular level. This will help develop new burn treatments, including regenerative medicine and tissue engineering.
The purpose of the work is to simulate an alkaline burn and study the features of regeneration of a burn wound resulting from exposure to alkali on the skin of rats.
Materials and methods of research.
The study was conducted on male mongrel white rats (Rattusnorvegicus) weighing 300 grams. Alkali was applied to the dorsal surface of the animals' bodies in compliance with the rules of working with chemicals, biopsy samples were taken on days 3, 7, 14 and 21, and micro-preparations were prepared with their subsequent study. At the same time, blood was taken from the femoral vein to obtain plasma and conduct a tesiographic examination [1, 2].
The results of the study. A change of regeneration phases from hemostasis to remodeling was noted on histological preparations. The tesiographic method recorded a change in the shape of crystals from hemispheroliths to spherulites and the number of crystal rays, which also corresponds to certain stages of healing, which are consistent with the picture on micro-preparations.
Conclusion. The skin was restored after exposure to alkali according to the standard wound healing scheme, which was confirmed by visual examination, measurement of the wound area, examination of tissue samples under a microscope, as well as by the method of tesiography.
About the authors
Dinara Jamaladinovna Khasanova
Tver State Medical University
Email: matakubovasirin26@gmail.com
ORCID iD: 0009-0002-7285-3021
Russian Federation, Tver, Sovetskaya street, 4
Ekaterina Andreevna Osipova
Tver State Medical University
Author for correspondence.
Email: osipovaekaterina2021@gmail.com
ORCID iD: 0009-0000-8217-575X
Russian Federation, Tver, Sovetskaya street, 4
Ekaterina Borisovna Ganina
Tver State Medical University
Email: katena-ganina@mail.ru
ORCID iD: 0000-0002-8686-8526
Candidate of Biological Sciences, Senior Lecturer at the Department of Anatomy, Histology and Embryology
Russian Federation, Tver, Sovetskaya street, 4Anna Olegovna Buglak
Tver State Medical University
Email: snej04ik@gmail.com
ORCID iD: 0009-0005-0534-7921
Senior Lecturer at the Department of Anatomy, Histology and Embryology
Russian Federation, Tver, Sovetskaya street, 4References
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