Applied Information Aspects of Medicine (Prikladnye informacionnye aspekty mediciny)

2070-9277

Voronezh State Medical University named after N.N. Burdenko - The State Budgetary Institution of Higher Professional Education «Voronezh State Medical University named after N.N. Burdenko» of the Ministry of Public Health of the Russian

9930

10.18499/2070-9277-2024-27-1-%p

THE EFFECT OF HIGH SUCCINATE CONCENTRATION ON MYOGENESIS OF THE C2C12 CELL LINE IN VITRO

Isaeva

Maria

mia.poroshina@yandex.ruAbalenikhina

Julia

abalenihina88@mail.ruGadzhieva

Fidan

fidagadzhiieva2003@gmail.comShchulkin

Alexey

alekseyshulkin@rambler.ruYakusheva

Elena

e.yakusheva@rzgmu.ru

31032024

2719299

04042024

2024

Myogenesis is a complex process that involves many stages, starting from the formation of precursor cells of muscle tissue and ending with the formation of mature muscle fibers. Succinate is an intermediate product of metabolism and metabolites of the tricarboxylic acid cycle (Krebs cycle). During myogenesis, it participates in the synthesis of mitochondrial proteins that provide cells with energy and is a donor of protons and electrons for the II complex of the respiratory chain (succinate dehydrogenase). The aim of the study is to study the effect of succinate at a concentration of 1 mM on the differentiation of the C2C12 cell line. Microscopic (assessment of cell length, width and myogenesis index) and Western blot methods (assessment of the transcription factor regulating the initial stages of differentiation - MyoD) were used for the study. The experiment showed that succinate at a concentration of 1 mM stimulates accelerated differentiation of myoblasts, increasing MyoD levels and stimulating the formation of myotubes in the first days of differentiation. Thus, succinate at a concentration of 1 mM can be considered as a promising drug for rapid stimulation of muscle tissue regeneration for athletes after heavy muscle loads and for astronauts during adaptation after a long stay in zero gravity.

C2C12 cell linemyogenesiscell fusion indexsuccinate.

клеточная линия С2С12миогенезиндекс слияния клетоксукцинат.

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