Medical Scientific Bulletin of Central Chernozemye (Naučno-medicinskij vestnik Centralʹnogo Černozemʹâ)Medical Scientific Bulletin of Central Chernozemye (Naučno-medicinskij vestnik Centralʹnogo Černozemʹâ)1990-472XФедеральное государственное бюджетное образовательное учреждение высшего образования "Воронежский государственный медицинский университет имени Н.Н. Бурденко" Министерства здравоохранения Российской Федерации790510.18499/1990-472X-2022-0-89-34-38Original ArticleANTIMICROBIAL ACTIVITY OF THE PIPTOPORUS BETULINUS WATER-ALCOHOL EXTRACTHarbatsevichH. I-Belarusian State University1512202289343828102022Copyright © 2022, Medical Scientific Bulletin of Central Chernozemye (Naučno-medicinskij vestnik Centralʹnogo Černozemʹâ)2022In this work, the antimicrobial activity of the Piptoporus betulinus water-alcohol extract fractions has been studied. The low-polarity fractions obtained using chloroform as an extractant demonstrated the highest antimicrobial activity against Staphylococcus aureus (6.7±0.8 mm), Serratia marcescens (14.9±0.4 mm) and Bacillus subtilis (9.8±0.5 mm), comparable to the action of antibiotics used in medicine. In accordance with the literature data, the antimicrobial effect can be related to the triterpene acids of the lanostane series (polyporenic acids A and C, 3β-acetoxy-16α-hydroxy-24-oxo-5α-lanosta-8-en-21-oic acid) and the antibiotic piptamine. The results are useful for developing a technology for obtaining purified extracts of Piptoporus betulinus, as potential antimicrobial agents.Piptoporus betulinuswater-alcohol extractantimicrobial activityтрутовик березовыйводно-спиртовой экстрактантмикробная активность1.Alresly, Zeyad. Chemical and Pharmacological Investigations of Fomitopsis betulina (formerly: Piptoporus betulinus) and Calvatia gigantea / Zeyad Alresly. - Inauguraldissertation. - Greifswald, 2019. - 241 p.2.Grienke, Ulrike.European medicinal polypores - A modern view on traditional uses / Ulrike Grienke, MargitZöll [et al.] // Journal of Ethnopharmacology. - 2014. - Vol. 154, № 3. - P. 564 - 583.3.Sułkowska-Ziaja, Katarzyna. Chemical composition and biological activity of extracts from fruiting bodies and mycelial cultures of Fomitopsis betulina / Katarzyna Sułkowska-Ziaja, Agnieszka Szewczyk [et al.] // Molecular Biology Reports. - 2018. - Vol. 45. - P. 2535 - 2544.4.Pleszczyńska, Małgorzata. Fomitopsis betulina (formerly Piptoporus betulinus): the Iceman’s polypore fungus with modern biotechnological potential / Małgorzata Pleszczyńska [et al.] // World Journal of Microbiology&Biotechnology. - 2017. - Vol. 33. - P. 83 - 94.5.Alresly, Zeyad. Bioactive Triterpenes from the Fungus Piptoporus betulinus / Zeyad Alresly,Ulrike Lindequist [et al.] // Records of natural products. - 2016. - Vol. 10, № 1. - P. 103 - 108.6.Balouiri M. Methods for in vitro evaluating antimicrobial activity: A review / M. Balouiri, M. Sadiki, S.K. Ibnsouda // Journal of Pharmaceutical Analysis. - 2016. - Vol. 6. - № 2. - P. 71.7.Kawagishi, Hirokazu. Novel Hydroquinone as a Matrix Metallo-proteinase Inhibitor from the Mushroom, Piptoporus betulinus / Hirokazu Kawagishi // Bioscience, Biotechnology, and Biochemistry. - 2002. - Vol. 66, № 12. - P. 2748 - 2750.8.Chemical characterization and wound healing property of a β-D-glucan from edible mushroom Piptoporus betulinus / L.I. de Jesus [et al.] // International Journal of Biological Macromolecules. - 2018. - Vol. 117. - P. 1361-1366.